Another problem a player may face is a [[Overcoming_Obstacles#Misalignments | misalignment]]. Sometimes the slides become misaligned and cause a break in the 2D image. When faced with this challenge a player should look for clues like branch width and direction to help them figure out where the continuation may be.
玩家可能遇到的另外一个问题是 断层。由于载玻片的偏移,就毁了二维切片图。玩家应该能找到分支的大小和方向灯线索去追踪下一部分可能在哪里。