Difference between revisions of "CB2-RGC"

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(Molecular Definition)
(Molecular Definition)
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==Molecular Definition==
 
==Molecular Definition==
These retinal ganglion cells are defined by their expression of calretinin (28 kDa calbindin). They can be visualized in calretinin-EGFP mice (CB2-GFP mice), in which GFP is expressed under the calretinin promoter (GENSAT).
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These retinal ganglion cells are defined by their expression of calretinin (28 kDa calbindin). They can be visualized in calretinin-EGFP BAC transgenic mice (CB2-GFP mice), in which GFP is expressed under the calbindin 2 promoter (GENSAT). Although CB2-RGCs are the only cells in the ganglion cell layer (GCL) that express GFP in this mouse, it is also expressed by a group of amacrine cells in the inner nuclear layer (INL).
  
 
==Physiology==
 
==Physiology==

Revision as of 16:22, 25 September 2015

Retinal ganglion cells that express calretinin, or 29 kDa calbindin, are known as CB2-GRCs. They are a type of transient OFF-alpha RGCs (tOFF-alphaRGC). Like all alpha cells, these RGCs are immunoreactive with SMI-32, an antibody that recognizes an antigen rich in alpha RGC neurofilaments. They possess large, spherical somas, and monostratify within the OFF sublamina of the inner nuclear layer (IPL). Typical of cells that exhibit transient OFF-type physiological responses, they depolarize and spike briefly at the offset of a flash (Huberman et al., 2008). Their axons project exclusively to the superior colliculus (SC) and the dorsal lateral geniculate nucleus (dLGN).

Molecular Definition

These retinal ganglion cells are defined by their expression of calretinin (28 kDa calbindin). They can be visualized in calretinin-EGFP BAC transgenic mice (CB2-GFP mice), in which GFP is expressed under the calbindin 2 promoter (GENSAT). Although CB2-RGCs are the only cells in the ganglion cell layer (GCL) that express GFP in this mouse, it is also expressed by a group of amacrine cells in the inner nuclear layer (INL).

Physiology

Anatomy

Dendritic Morphology

Retinal Input

Central Projections

Behavioral Output

History

References

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